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hydroxyapatite bio gel htp gel slurry  (Bio-Rad)


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    Structured Review

    Bio-Rad hydroxyapatite bio gel htp gel slurry
    Hydroxyapatite Bio Gel Htp Gel Slurry, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 801 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hydroxyapatite+slurry/Bio-Gel+HTP+Hydroxyapatite/pm41411413-266-19-24
    Average 94 stars, based on 801 article reviews
    hydroxyapatite bio gel htp gel slurry - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Retinoic acid X receptor in the diploblast, Tripedalia cystophora
    Article Snippet: Either 10 or 20 ng of (His) 6 jRXR fusion protein was incubated on ice for 3 hr with labeled retinoids at variable concentration with or without a 100 fold excess of unlabeled competitors in binding buffer [50 mM Tris⋅HCl, pH 7.4/1 mM EDTA/5 mM dithiotreitol/120 mM KCl, 8% (vol/vol) glycerol/0.5% (wt/vol) 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate/1 mM phenylmethylsulfonyl fluoride/10 ng/ml soybean trypsin inhibitor/2 μM E64/1.6 mM benzamidine/1 μM pepstatin A/5 μg/ml of leupeptin (all inhibitors from Boehringer Mannheim)] in final volumes of 500 μl. .. Proteins were separated from free ligands with a hydroxyapatite slurry (Ceramic Hydroxyapatite, Bio-Rad) as described ( 25 ) and washed 3 times in 1 ml of ice cold wash solution [50 mM Tris⋅HCl, pH 7.4/1.5 mM EDTA/120 mM KCl/0.5% (wt/vol) 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate with intervening centrifugation (5 min at 1,100 × g ). .. Total radioactivity was estimated by scintillation beta detector (Beckman) from aliquots of samples taken during the incubation, bound radioactivity was measured by transferring the final pelleted hydroxyapatite-protein fraction resuspended in 400 μl of ethanol to scintillation solution and free ligands were estimated from collected wash fractions.

    Article Title: Trichoplax adhaerens reveals a network of nuclear receptors sensitive to 9- cis -retinoic acid at the base of metazoan evolution
    Article Snippet: For estimation of specific binding, 200x excess of either 9- cis- RA or AT-RA (Sigma-Aldrich) was used. .. In case of GST-RXR fusion protein, 50 μl of the supernatant was removed after 30 s at 1300 g and washed 3x with 1000 μl wash buffer (50 mM Tris–HCl pH7.4, 1 mM EDTA, 120 mM KCl, 5 mM DTT, 8% (v/v) glycerol, 0.5% (w/v) CHAPS) removing 900 μl after each wash. For cleaved RXR protein 10 μl hydroxyapatite slurry (AG-1 XB Resin, Bio-Rad, Hercules, CA, USA) suspended in binding buffer (12.7 mg/100 μl) were added to the assay and mixed twice, collecting the apatite slurry by centrifugation (15 s at 600 g). .. 95 μl of the supernatant was removed and the slurry washed twice with 1 ml of wash buffer, removing 900 μl after each wash. Work with retinoids was done under indirect illumination with a 60 W, 120 V yellow light bulb (BugLite, General Electric Co, Nela Parc, Cleveland Oh, USA) as described ( ).

    Binding Assay:

    Article Title: Trichoplax adhaerens reveals a network of nuclear receptors sensitive to 9- cis -retinoic acid at the base of metazoan evolution
    Article Snippet: For estimation of specific binding, 200x excess of either 9- cis- RA or AT-RA (Sigma-Aldrich) was used. .. In case of GST-RXR fusion protein, 50 μl of the supernatant was removed after 30 s at 1300 g and washed 3x with 1000 μl wash buffer (50 mM Tris–HCl pH7.4, 1 mM EDTA, 120 mM KCl, 5 mM DTT, 8% (v/v) glycerol, 0.5% (w/v) CHAPS) removing 900 μl after each wash. For cleaved RXR protein 10 μl hydroxyapatite slurry (AG-1 XB Resin, Bio-Rad, Hercules, CA, USA) suspended in binding buffer (12.7 mg/100 μl) were added to the assay and mixed twice, collecting the apatite slurry by centrifugation (15 s at 600 g). .. 95 μl of the supernatant was removed and the slurry washed twice with 1 ml of wash buffer, removing 900 μl after each wash. Work with retinoids was done under indirect illumination with a 60 W, 120 V yellow light bulb (BugLite, General Electric Co, Nela Parc, Cleveland Oh, USA) as described ( ).



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